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rabbit hrp conjugated secondary antibodies  (Bio-Rad)


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    Structured Review

    Bio-Rad rabbit hrp conjugated secondary antibodies
    Rabbit Hrp Conjugated Secondary Antibodies, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 4904 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/secondary+antibody+goat+anti+rabbit+igg/Goat+Anti-Mouse+IgG+(H+%2B+L)-HRP+Conjugate/pmc13659450-207-2-13
    Average 99 stars, based on 4904 article reviews
    rabbit hrp conjugated secondary antibodies - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Membrane:

    Article Title: Effects of corticosteroid on mRNA levels of histamine H1 receptor in nasal mucosa of healthy participants and HeLa cells.
    Article Snippet: The membrane was then incubated overnight at 4°C with one of the following primary antibodies : PKCδ (C-20), sc-937, 1:1000 ; ERK (K-23), sc-94, 1:1000 ; phospho-ERK (E-4), sc-7383, 1:1000, (Santa Cruz Biotechnology) ; phospho-PKCδ (Tyr311), #2055S, 1:500 (Cell Signaling). .. The membrane was then incubated in the appropriate secondary antibody (goat anti-rabbit IgG (H ± L)-HRP conjugate (#170-6515, 1:10,000, Bio-Rad) or Immun-Star goat anti-mouse-HRP conjugate (#170-5047, 1:10,000, Bio-Rad) and proteins visualized using Immobilon Western Chemiluminescent HRP substrate (Millipore). ..

    Article Title: Social Isolation Induces Neuroinflammation And Microglia Overactivation, While Dihydromyricetin Prevents And Improves Them
    Article Snippet: The membrane was incubated in the following primary antibody for either: rabbit anti-mouse Gephyrin (Cell Signaling 14304, 1:1000), mouse anti-mouse β-actin (Cell Signaling 4970, 1:1000), rabbit anti-mouse NF-κB p65 (Cell Signaling 8242, 1:1000) or rabbit anti-mouse Phospho-NF-κB p65 (Cell Signaling 3033, 1:1000) in 1X TBST overnight at 4°C with gentle agitation. .. The membrane was washed three times with 1X TBST for 10 min each and incubated with a secondary antibody goat anti-rabbit IgG or goat anti-mouse (Bio-Rad 1706515 and 1706516) in 1X TBST for 1 hour. .. Finally, the membrane was visualized with enhanced chemiluminescence detection reagent (Bio-Rad 1705061) and Chemi-Doc (Bio-Rad) imaging device.

    Article Title: Social isolation induces neuroinflammation and microglia overactivation, while dihydromyricetin prevents and improves them
    Article Snippet: The membrane was incubated in the following primary antibody for either: rabbit anti-mouse gephyrin (Cell Signaling 14304, 1:1000), mouse anti-mouse β-actin (Cell Signaling 4970, 1:1000), rabbit anti-mouse NF-κB p65 (Cell Signaling 8242, 1:1000) or rabbit anti-mouse phospho-NF-κB p65 (Cell Signaling 3033, 1:1000) in 1X TBST overnight at 4 °C with gentle agitation. .. The membrane was washed three times with 1X TBST for 10 min each and incubated with a secondary antibody goat anti-rabbit IgG or goat anti-mouse (Bio-Rad 1706515 and 1706516) in 1X TBST for 1 h. Finally, the membrane was visualized with enhanced chemiluminescence detection reagent (Bio-Rad 1705061) and Chemi-Doc (Bio-Rad) imaging device. ..

    Incubation:

    Article Title: Effects of corticosteroid on mRNA levels of histamine H1 receptor in nasal mucosa of healthy participants and HeLa cells.
    Article Snippet: The membrane was then incubated overnight at 4°C with one of the following primary antibodies : PKCδ (C-20), sc-937, 1:1000 ; ERK (K-23), sc-94, 1:1000 ; phospho-ERK (E-4), sc-7383, 1:1000, (Santa Cruz Biotechnology) ; phospho-PKCδ (Tyr311), #2055S, 1:500 (Cell Signaling). .. The membrane was then incubated in the appropriate secondary antibody (goat anti-rabbit IgG (H ± L)-HRP conjugate (#170-6515, 1:10,000, Bio-Rad) or Immun-Star goat anti-mouse-HRP conjugate (#170-5047, 1:10,000, Bio-Rad) and proteins visualized using Immobilon Western Chemiluminescent HRP substrate (Millipore). ..

    Article Title: Social Isolation Induces Neuroinflammation And Microglia Overactivation, While Dihydromyricetin Prevents And Improves Them
    Article Snippet: The membrane was incubated in the following primary antibody for either: rabbit anti-mouse Gephyrin (Cell Signaling 14304, 1:1000), mouse anti-mouse β-actin (Cell Signaling 4970, 1:1000), rabbit anti-mouse NF-κB p65 (Cell Signaling 8242, 1:1000) or rabbit anti-mouse Phospho-NF-κB p65 (Cell Signaling 3033, 1:1000) in 1X TBST overnight at 4°C with gentle agitation. .. The membrane was washed three times with 1X TBST for 10 min each and incubated with a secondary antibody goat anti-rabbit IgG or goat anti-mouse (Bio-Rad 1706515 and 1706516) in 1X TBST for 1 hour. .. Finally, the membrane was visualized with enhanced chemiluminescence detection reagent (Bio-Rad 1705061) and Chemi-Doc (Bio-Rad) imaging device.

    Article Title: Social isolation induces neuroinflammation and microglia overactivation, while dihydromyricetin prevents and improves them
    Article Snippet: The membrane was incubated in the following primary antibody for either: rabbit anti-mouse gephyrin (Cell Signaling 14304, 1:1000), mouse anti-mouse β-actin (Cell Signaling 4970, 1:1000), rabbit anti-mouse NF-κB p65 (Cell Signaling 8242, 1:1000) or rabbit anti-mouse phospho-NF-κB p65 (Cell Signaling 3033, 1:1000) in 1X TBST overnight at 4 °C with gentle agitation. .. The membrane was washed three times with 1X TBST for 10 min each and incubated with a secondary antibody goat anti-rabbit IgG or goat anti-mouse (Bio-Rad 1706515 and 1706516) in 1X TBST for 1 h. Finally, the membrane was visualized with enhanced chemiluminescence detection reagent (Bio-Rad 1705061) and Chemi-Doc (Bio-Rad) imaging device. ..

    Article Title: Dihydromyricetin ameliorates social isolation-induced anxiety by modulating mitochondrial function, antioxidant enzymes, and BDNF.
    Article Snippet: Membranes were then incubated overnight (4 ◦C) with either mouse anti-mouse OxPhos antibody cocktail (Thermo Fisher 45–8099, 1:200), mouse anti-mouse β-actin (Cell Signaling 4970, 1:1000), rabbit anti-mouse (mAb) SOD-2 (Cell Signaling 13141, 1:1000), rabbit mAb HO-1 (Cell Signaling 86806, 1:1000), rabbit mAb PRDX-3 (Abcam ab73349, 1:1000), rabbit mAb GPX-4 (Cell Signaling 52455, 1:1000), Bcl-1 rabbit mAb Cell Signaling 42406, 1:1000), p62 rabbit mAb Cell Signaling 23214, 1:1000), BDNF (Cell Signaling 47808, 1:1000), p-TrkB (Abcam ab229908, 1:1000), p44/42 MAPK (Erk1/2) (Cell Signaling L34F12, 1:1000), Phospho-p44/42 MAPK (Erk1/2) (Cell Signaling D13.14.4E, 1:1000). .. After washing three times with 1X TBST, the membranes were incubated in a secondary antibody goat anti-rabbit IgG or goat anti- mouse (Bio-Rad 1706515 and 1706516) in 1X TBST for 1 h. Blots were subsequently developed with an enhanced chemiluminescence (ECL) system (Bio-Rad 1705061) and visualized using a Chemi-Doc (BioRad) imaging device. .. The colorimetric superoxide dismutase activity assay kit (ab65354, Abcam, Cambridge, MA, USA) was used to detect the activity of SOD in PFC homogenates.

    Article Title: Dihydromyricetin ameliorates social isolation-induced anxiety by modulating mitochondrial function, antioxidant enzymes, and BDNF
    Article Snippet: Membranes were then incubated overnight (4 °C) with either mouse anti-mouse OxPhos antibody cocktail (Thermo Fisher 45–8099, 1:200), mouse anti-mouse β-actin (Cell Signaling 4970, 1:1000), rabbit anti-mouse (mAb) SOD-2 (Cell Signaling 13141, 1:1000), rabbit mAb HO-1 (Cell Signaling 86806, 1:1000), rabbit mAb PRDX-3 (Abcam ab73349, 1:1000), rabbit mAb GPX-4 (Cell Signaling 52455, 1:1000), Bcl-1 rabbit mAb Cell Signaling 42406, 1:1000), p62 rabbit mAb Cell Signaling 23214, 1:1000), BDNF (Cell Signaling 47808, 1:1000), p-TrkB (Abcam ab229908, 1:1000), p44/42 MAPK (Erk1/2) (Cell Signaling L34F12, 1:1000), Phospho-p44/42 MAPK (Erk1/2) (Cell Signaling D13.14.4E, 1:1000). .. After washing three times with 1X TBST, the membranes were incubated in a secondary antibody goat anti-rabbit IgG or goat anti-mouse (Bio-Rad 1706515 and 1706516) in 1X TBST for 1 h. Blots were subsequently developed with an enhanced chemiluminescence (ECL) system (Bio-Rad 1705061) and visualized using a Chemi-Doc (Bio-Rad) imaging device. .. The colorimetric superoxide dismutase activity assay kit (ab65354, Abcam, Cambridge, MA, USA) was used to detect the activity of SOD in PFC homogenates.

    Western Blot:

    Article Title: Effects of corticosteroid on mRNA levels of histamine H1 receptor in nasal mucosa of healthy participants and HeLa cells.
    Article Snippet: The membrane was then incubated overnight at 4°C with one of the following primary antibodies : PKCδ (C-20), sc-937, 1:1000 ; ERK (K-23), sc-94, 1:1000 ; phospho-ERK (E-4), sc-7383, 1:1000, (Santa Cruz Biotechnology) ; phospho-PKCδ (Tyr311), #2055S, 1:500 (Cell Signaling). .. The membrane was then incubated in the appropriate secondary antibody (goat anti-rabbit IgG (H ± L)-HRP conjugate (#170-6515, 1:10,000, Bio-Rad) or Immun-Star goat anti-mouse-HRP conjugate (#170-5047, 1:10,000, Bio-Rad) and proteins visualized using Immobilon Western Chemiluminescent HRP substrate (Millipore). ..

    Saline:

    Article Title: Rapid and sensitive determination of protein-nitrotyrosine by ELISA: Application to human plasma.
    Article Snippet: 3-Nitrotyrosine (3NT) is known as an important indicator of nitrosative stress and has been linked to various diseases.. Our aim was to develop an indirect ELISA (enzyme-linked immunosorbent assay) method suitable for the detection of proteinbound 3NT in clinical plasma and serum samples.. Nitrated protein standards and reduced protein standards were prepared.

    Imaging:

    Article Title: Social isolation induces neuroinflammation and microglia overactivation, while dihydromyricetin prevents and improves them
    Article Snippet: The membrane was incubated in the following primary antibody for either: rabbit anti-mouse gephyrin (Cell Signaling 14304, 1:1000), mouse anti-mouse β-actin (Cell Signaling 4970, 1:1000), rabbit anti-mouse NF-κB p65 (Cell Signaling 8242, 1:1000) or rabbit anti-mouse phospho-NF-κB p65 (Cell Signaling 3033, 1:1000) in 1X TBST overnight at 4 °C with gentle agitation. .. The membrane was washed three times with 1X TBST for 10 min each and incubated with a secondary antibody goat anti-rabbit IgG or goat anti-mouse (Bio-Rad 1706515 and 1706516) in 1X TBST for 1 h. Finally, the membrane was visualized with enhanced chemiluminescence detection reagent (Bio-Rad 1705061) and Chemi-Doc (Bio-Rad) imaging device. ..

    Article Title: Dihydromyricetin ameliorates social isolation-induced anxiety by modulating mitochondrial function, antioxidant enzymes, and BDNF.
    Article Snippet: Membranes were then incubated overnight (4 ◦C) with either mouse anti-mouse OxPhos antibody cocktail (Thermo Fisher 45–8099, 1:200), mouse anti-mouse β-actin (Cell Signaling 4970, 1:1000), rabbit anti-mouse (mAb) SOD-2 (Cell Signaling 13141, 1:1000), rabbit mAb HO-1 (Cell Signaling 86806, 1:1000), rabbit mAb PRDX-3 (Abcam ab73349, 1:1000), rabbit mAb GPX-4 (Cell Signaling 52455, 1:1000), Bcl-1 rabbit mAb Cell Signaling 42406, 1:1000), p62 rabbit mAb Cell Signaling 23214, 1:1000), BDNF (Cell Signaling 47808, 1:1000), p-TrkB (Abcam ab229908, 1:1000), p44/42 MAPK (Erk1/2) (Cell Signaling L34F12, 1:1000), Phospho-p44/42 MAPK (Erk1/2) (Cell Signaling D13.14.4E, 1:1000). .. After washing three times with 1X TBST, the membranes were incubated in a secondary antibody goat anti-rabbit IgG or goat anti- mouse (Bio-Rad 1706515 and 1706516) in 1X TBST for 1 h. Blots were subsequently developed with an enhanced chemiluminescence (ECL) system (Bio-Rad 1705061) and visualized using a Chemi-Doc (BioRad) imaging device. .. The colorimetric superoxide dismutase activity assay kit (ab65354, Abcam, Cambridge, MA, USA) was used to detect the activity of SOD in PFC homogenates.

    Article Title: Dihydromyricetin ameliorates social isolation-induced anxiety by modulating mitochondrial function, antioxidant enzymes, and BDNF
    Article Snippet: Membranes were then incubated overnight (4 °C) with either mouse anti-mouse OxPhos antibody cocktail (Thermo Fisher 45–8099, 1:200), mouse anti-mouse β-actin (Cell Signaling 4970, 1:1000), rabbit anti-mouse (mAb) SOD-2 (Cell Signaling 13141, 1:1000), rabbit mAb HO-1 (Cell Signaling 86806, 1:1000), rabbit mAb PRDX-3 (Abcam ab73349, 1:1000), rabbit mAb GPX-4 (Cell Signaling 52455, 1:1000), Bcl-1 rabbit mAb Cell Signaling 42406, 1:1000), p62 rabbit mAb Cell Signaling 23214, 1:1000), BDNF (Cell Signaling 47808, 1:1000), p-TrkB (Abcam ab229908, 1:1000), p44/42 MAPK (Erk1/2) (Cell Signaling L34F12, 1:1000), Phospho-p44/42 MAPK (Erk1/2) (Cell Signaling D13.14.4E, 1:1000). .. After washing three times with 1X TBST, the membranes were incubated in a secondary antibody goat anti-rabbit IgG or goat anti-mouse (Bio-Rad 1706515 and 1706516) in 1X TBST for 1 h. Blots were subsequently developed with an enhanced chemiluminescence (ECL) system (Bio-Rad 1705061) and visualized using a Chemi-Doc (Bio-Rad) imaging device. .. The colorimetric superoxide dismutase activity assay kit (ab65354, Abcam, Cambridge, MA, USA) was used to detect the activity of SOD in PFC homogenates.



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